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 Gene information |
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| Locus | FGSG_07583 | Length | 247 aa |
| Gene Name | GzNEG002 | # Motifs | 1 |
| Family-1 | Negative transcriptional regulator | Deletion | Y |
| Family-2 | Parent strain | GZ3639 | |
| Family-3 | Reference | ||
| Primers |
S o u t h e r n b l o t |
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|
| name | sequence | ||
| 5F | TTCCAGCGCACAACACAGACCA | ||
| 5R | gcacaggtacacttgtttagagATGGATGAGACGACGAAGGACACG | ||
| 3F | ccttcaatatcatcttctgtcgTCGTACGGCTCAGTGGAAGGATTA | ||
| 3R | TGATGTCAGGCCACGGAGAGAC | ||
| 5N | ATCCGCCTTCCAACTCACCACTGT | ||
| 3N | GCCGGAATGTGATGCAATAGGACT | ||
| with 5F | AATGCTCTATTATCGCTTTGCTCTGG | ||
| Phenotypes |
Wild type | Mutant | |
| Mycelia growth | PDA | No image | No image |
| MM | No image | No image | |
| Sexual development | Number of perithecia (NP) | No image | No image |
| Perithecia maturation (PM) | |||
| Ascospore formation (AF) | No image | No image | |
| Ascospore discharge (AD) | |||
| Conidiation (106) | |||
| Toxin Production | No image | No image | |
| Virulence | No image | No image | |
| Stress responses | |||
| No image | |||
FGSG_07583 - GzNEG002