|
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
 Gene information |
|||
| Locus | FGSG_08481 | Length | 1,106 aa |
| Gene Name | GzWing018 | # Motifs | 1 |
| Family-1 | Winged helix repressor DNA-binding | Deletion | Y |
| Family-2 | Parent strain | GZ3639 | |
| Family-3 | Reference | ||
| Primers |
S o u t h e r n b l o t |
 |
|
| name | sequence | ||
| 5F | TGAGACAGAGATTACAACCAACGGAAC | ||
| 5R | gcacaggtacacttgtttagagATTGTGGGGGTGCCGTGTATT | ||
| 3F | ccttcaatatcatcttctgtcgGACCCAAGAGCTCATCAAAGAAAAAG | ||
| 3R | AGCAGACCTAAAGCTTTAGCACAGAAGT | ||
| 5N | GCCCATTCTACAGGCCTTGCTATTT | ||
| 3N | TGGAAGAGGGTACAAATATCGTTGAGG | ||
| with 5F | CAGATTTCTAAAGCAAGCGACACTCAC | ||
| Phenotypes |
Wild type | Mutant | |
| Mycelia growth | PDA |  |
 |
| MM |  |
 |
|
| Sexual development | Number of perithecia (NP) |  |
 |
| Perithecia maturation (PM) | |||
| Ascospore formation (AF) | No image | No image | |
| Ascospore discharge (AD) | |||
| Conidiation (106) | 2.62 | 0.31 | |
| Toxin Production |  |
 |
|
| Virulence |  |
 |
|
| Stress responses | |||
 |
|||
FGSG_08481 - GzWing018