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 Gene information |
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| Locus | FGSG_08981 | Length | 459 aa |
| Gene Name | GzOpi | # Motifs | |
| Family-1 | Transcription factor Opi1 | Deletion | Y |
| Family-2 | Parent strain | GZ3639 | |
| Family-3 | Reference | ||
| Primers |
S o u t h e r n b l o t |
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| name | sequence | ||
| 5F | CTTACGTTGCTTGGGCGGTGT | ||
| 5R | gcacaggtacacttgtttagagCCGAGGGGATGGCGAGACT | ||
| 3F | ccttcaatatcatcttctgtcgCCAGTGTTGCCTCAAACAGAGTCC | ||
| 3R | TCCAGCAGCATTTATACCACTTCTTC | ||
| 5N | GCCTGCGCCTCACCTGTCTG | ||
| 3N | CGCATCAATCGTTTTACCCACC | ||
| with 5F | GATTCGAATAAGCTGGAGGGGATAG | ||
| Phenotypes |
Wild type | Mutant | |
| Mycelia growth | PDA | No image | No image |
| MM |  |
 |
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| Sexual development | Number of perithecia (NP) | No image | No image |
| Perithecia maturation (PM) | |||
| Ascospore formation (AF) | No image | No image | |
| Ascospore discharge (AD) | |||
| Conidiation (106) | 1.20 | 0.38 | |
| Toxin Production | No image | No image | |
| Virulence | No image | No image | |
| Stress responses | |||
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FGSG_08981 - GzOpi